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. 2008 Mar;5(1):55-9.
doi: 10.1093/ecam/nel077.

Inhibitory Response of Raphanus sativus on Lipid Peroxidation in Albino Rats

P Chaturvedi  1
Affiliations

Inhibitory Response of Raphanus sativus on Lipid Peroxidation in Albino Rats

P Chaturvedi. Evid Based Complement Alternat Med. 2008 Mar.

Abstract

In the present study, inhibitory effect of the methanol extract of Raphanus sativus root on lipid peroxidation has been carried out in normal rats. Graded doses of methanol extract of root of the plant (40, 80 and 120 mg kg(-1) body weight) were administered orally for 15 days to experimental treated rats. Distilled water was administered to experimental control rats. At the end of experiment, rats were killed by decapitation after ether anesthesia. Blood and liver were collected to measure thiobarbituric acid reactive substance, reduced glutathione and activity of catalase. Results indicated that the extract of R. sativus root reduced the levels of thiobarbituric acid reactive substance significantly in all experimental treated groups (P < 0.05) as compared to the experimental control group. It also increased the levels of reduced glutathione and increased the activity of catalase. In vitro experiments with the liver of experimental control and experimental treated rats were also carried out against cumene hydroperoxide induced lipid peroxidation. The extract inhibited in vitro cumene hydroperoxide induced lipid peroxidation. R. sativus inhibits lipid peroxidation in vivo and in vitro. It provides protection by strengthening the antioxidants like glutathione and catalase. Inclusion of this plant in every day diet would be beneficial.

Keywords: Raphanus sativus; catalase; glutathione; lipid peroxidation; thiobarbituric acid reactive substance.

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Figures

Figure 1.
Figure 1.
In vitro effect of R. sativus root extract on TBARS in experimental control and experimental treated rat liver homogenate in CHP-induced lipid peroxidation. NC: homogenate from experimental control rats + distilled water + CHP, NE: homogenate from experimental control rats + extract + CHP, E3C: homogenate from experimental treated rats (received 120 mg kg−1 body weight)+distilled water+CHP, E3E: homogenate from experimental treated rats (received 120 mg kg−1 body weight) + extract + CHP. N = 5 in all groups, P < 0.05 when NE and E3C compared with NC at 10, 20 and 40 min, P < 0.01 when E3E compared with NC at 10, 20 and 40 min.
Figure 2.
Figure 2.
In vitro effect of R. sativus root extract on GSH in experimental control and experimental treated rat liver homogenate in CHP-induced lipid peroxidation. NC: homogenate from experimental control rats + distilled water + CHP, NE: homogenate from experimental control rats + extract + CHP, E3C: homogenate from experimental treated rats (received 120 mg kg−1 body weight) + distilled water + CHP, E3E: homogenate from experimental treated rats (received 120 mg kg−1 body weight) + extract + CHP. N = 5 in all groups, P < 0.05 when NE, E3E and E3C compared with NC at 20 and 40 min.
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References

    1. Yu BP. Cellular defence against damage from reactive oxygen species. Physiol Rev. 1994;74:139–62. - PubMed
    1. Pierre SH, Georges AA, Simon G, Michel B. Natural health products, modulation of immune functions and prevention of chronic diseases. Evid Based Complement Alternat Med. 2005;4:513–20. - PMC - PubMed
    1. Khan KI, Khan FJ, Shahida K. Antimicrobial activity of Alum ceppa and Raphanus sativus. J Pharmacol. 1985;6:59–72.
    1. Chaturvedi P, Akala H. Effect of Raphanus sativus root extracts on glucose level in normal and diabetic rats. J Appl Zool Res. 2001;12:172–7.
    1. Sipos P, Hagymasi K, Lugasi A, Feher E, Blazovics A. Effect of black radish root (R. sativus L var niger) on the colon mucosa in rats fed a fat rich diet. Phytother Res. 2002;16:677–9. - PubMed

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