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. 2008 Mar 1;64(Pt 3):228-30.
doi: 10.1107/S1744309108004326. Epub 2008 Feb 29.

Crystallization and preliminary X-ray crystallographic analysis of rabbit L-gulonate 3-dehydrogenase

Yukuhiko Asada  1 , Chizu Kuroishi, Yoko Ukita, Rie Sumii, Satoshi Endo, Toshiyuki Matsunaga, Akira Hara, Naoki Kunishima
Affiliations

Crystallization and preliminary X-ray crystallographic analysis of rabbit L-gulonate 3-dehydrogenase

Yukuhiko Asada et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. .

Abstract

Rabbit L-gulonate 3-dehydrogenase was crystallized using the oil-microbatch method at 295 K. X-ray diffraction data were collected to 1.70 A resolution from a crystal at 100 K using synchrotron radiation. The crystal belongs to the C-centred monoclinic space group C2, with unit-cell parameters a = 71.81, b = 69.08, c = 65.64 A, beta = 102.7 degrees. Assuming the presence of a monomeric protomer in the asymmetric unit gives a V(M) value of 2.21 A(3) Da(-1) and a solvent content of 44.4%. A cocrystal with NADH, which was isomorphous to the apo form, was also prepared and diffraction data were collected to 1.85 A resolution using Cu Kalpha radiation at 100 K.

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Figures

Figure 1
Figure 1
Crystals of rabbit GDH apoenzyme. The scale bar represents 0.2 mm.
See this image and copyright information in PMC

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