Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006 Jun;6(2):508-510.
doi: 10.1111/j.1471-8286.2006.01297.x.

Characterization of di-, tri-, and tetranucleotide microsatellite markers with perfect repeats for Trypanosoma brucei and related species

Oliver Balmer  1 , Christopher Palma, Annette Macleod, Adalgisa Caccone
Affiliations

Characterization of di-, tri-, and tetranucleotide microsatellite markers with perfect repeats for Trypanosoma brucei and related species

Oliver Balmer et al. Mol Ecol Notes. 2006 Jun.

Abstract

Trypanosoma brucei, unicellular parasites causing human sleeping sickness and animal nagana, have a great impact on the socio-economic environment of sub-Saharan Africa. The dynamics of the parasite are still poorly understood. We have characterized 14 polymorphic di-, tri-, and tetranucleotide microsatellite loci with perfect repeats (only one motif) exhibiting between 5 and 16 alleles in T. brucei isolates from all over Africa and from all described subspecies. The microsatellites will be useful in addressing population genetic questions in T. brucei to better understand the population structure and spread of this important parasite.

PubMed Disclaimer

References

    1. Applied Biosystems . GeneMapper Software Version 3.0. Applied Biosystems; 2002.
    1. Benson G. Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Research. 1999;27:573–580. - PMC - PubMed
    1. Biteau N, Bringaud F, Gibson W, Truc P, Baltz T. Characterization of Trypanozoon isolates using a repeated coding sequence and microsatellite markers. Molecular and Biochemical Parasitology. 2000;105:187–202. - PubMed
    1. Desquesnes M, Davila AMR. Applications of PCR-based tools for detection and identification of animal trypanosomes: a review and perspectives. Veterinary Parasitology. 2002;109:213–231. - PubMed
    1. MacLeod A, Tweedie A, McLellan S, et al. The genetic map and comparative analysis with the physical map of Trypanosoma brucei. Nucleic Acids Research. in press. - PMC - PubMed

LinkOut - more resources