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. 2008 May;15(5):891-2.
doi: 10.1128/CVI.00012-08. Epub 2008 Mar 12.

Prevention of assay interference in infectious-disease serology tests done on the liaison platform

Mario Berth  1 , Eugene Bosmans
Affiliations

Prevention of assay interference in infectious-disease serology tests done on the liaison platform

Mario Berth et al. Clin Vaccine Immunol. 2008 May.

Abstract

Immunoassay interference causing unexpected reactive results in magnetic-microparticle-based assays was detected. A systematic evaluation of Liaison Epstein-Barr virus immunoglobulin M showed that 5% of the positive results (0.4% of tested samples) could be explained by such interference. Adding chemical blocking reagents (polyvinylpyrrolidone and polyvinyl alcohol) to the assay buffers partially prevented this phenomenon.

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Figures

FIG. 1.
FIG. 1.
Bland-Altman plot comparing results obtained with the modified and original EBV IgM assays on the Liaison platform. The six discrepant results are marked (box). Only results of <400 mU/liter are shown (n = 89).
See this image and copyright information in PMC

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