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. 2008 May;52(5):1790-3.
doi: 10.1128/AAC.01440-07. Epub 2008 Mar 17.

Multiclonal outbreak of Klebsiella pneumoniae producing extended-spectrum beta-lactamase CTX-M-2 and novel variant CTX-M-59 in a neonatal intensive care unit in Brazil

Doroti de Oliveira Garcia  1 , Yohei Doi, Dora Szabo, Jennifer M Adams-Haduch, Tânia M I Vaz, Daniela Leite, Maria Clara Padoveze, Maristela P Freire, Fernanda P Silveira, David L Paterson
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Multiclonal outbreak of Klebsiella pneumoniae producing extended-spectrum beta-lactamase CTX-M-2 and novel variant CTX-M-59 in a neonatal intensive care unit in Brazil

Doroti de Oliveira Garcia et al. Antimicrob Agents Chemother. 2008 May.

Abstract

An outbreak of cephalosporin-resistant Klebsiella pneumoniae occurred in a neonatal intensive care unit in São Paulo, Brazil. Of the 10 pulsotypes identified during the outbreak and follow-up periods, nine produced CTX-M-2 or its new variant CTX-M-59 and one produced SHV-5. bla(CTX-M-2/59) genes were located on closely related plasmids that were transferable.

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Figures

FIG. 1.
FIG. 1.
PFGE profiles of the 10 pulsotypes identified in the study. M, marker.
FIG. 2.
FIG. 2.
DNA hybridization with a blaCTX-M-2-specific probe. blaCTX-M-2/59 contains a PstI restriction site. All the plasmids hybridized with the probe, except for that from pulsotype D, which is blaCTX-M-2/59 negative and blaSHV-5 positive.
See this image and copyright information in PMC

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