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Comparative Study
. 2008 Spring;13(1):67-71.
doi: 10.1007/s12192-008-0009-5. Epub 2008 Feb 6.

Alpha-hemoglobin-stabilizing protein (AHSP) in hemolysis, elevated liver enzyme, and low platelet (HELLP) syndrome, intrauterine growth restriction (IUGR) and fetal death

Monica Emanuelli  1 , Davide Sartini, Valentina Rossi, Alessandra Corradetti, Beatrice Landi, Claudia Regina Vianna, Stefano Giannubilo, Andrea Luigi Tranquilli
Affiliations
Comparative Study

Alpha-hemoglobin-stabilizing protein (AHSP) in hemolysis, elevated liver enzyme, and low platelet (HELLP) syndrome, intrauterine growth restriction (IUGR) and fetal death

Monica Emanuelli et al. Cell Stress Chaperones. 2008 Spring.

Abstract

Objective: Alpha hemoglobin-stabilizing protein (AHSP) inhibits the production of reactive oxygen species in various cells, including erythrocytes. Reduced AHSP can mean reduced protection from stressors. Our objective was to investigate whether AHSP is involved in the response to stress in pregnancy.

Study design: Placentas were collected from normal term pregnancies (n = 10) and pregnancies complicated by HELLP (n = 10), intrauterine growth restriction (IUGR; n = 10) or fetal death (IUFD; n = 6). AHSP messenger RNA (mRNA) and protein were determined using real time quantitative polymerase chain reaction (PCR) and Western blot, respectively. All statistical analyses were performed by using the GraphPad Prism Software. Differences were considered significant at p < 0.05.

Results: Placental AHSP mRNA level in HELLP (4.16E10(-4) +/- 1.77) and IUFD (4.19E10(-4) +/- 3.37) were significantly decreased compared with controls (28.47E10(-4) +/- 14.86; p < 0.01), whereas levels in the IUGR group (7.55E10(-4) +/- 6.4) showed a trend toward being lower but the difference did not reach statistical significance. Western blot analysis results indicate a no significant increase of ASHP protein in the HELLP syndrome group and a significant decrease in the IUFD group compared with controls. There was no significant difference between the IUGR and control groups.

Conclusion: ASHP mRNA expression in the placenta is decreased in complicated pregnancies, and it may be involved in the pathogenic mechanisms leading to the adverse pregnancy outcome.

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Figures

Fig. 1
Fig. 1
Real-time quantitative PCR. AHSP mRNA levels in the placenta of patients with normal or pathological pregnancies. Results are expressed as mean ± SD
Fig. 2
Fig. 2
Representative Western blot for AHSP. Protein lysate from placentas are shown on the first four lanes. The last lane shows the protein lysate from BL21 after induction of AHSP expression (positive control). The control lane shows the protein lysate that was present in all Western blots and used for normalization of expression
Fig. 3
Fig. 3
Western blot data analysis. Expression of AHSP protein in placentas of patients with normal or pathological pregnancies. Results are expressed as mean ± SD. *p < 0.05
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