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Comparative Study
. 2008 May;149(2):246-50.
doi: 10.1016/j.jviromet.2008.01.032. Epub 2008 Mar 18.

A multiplex TaqMan PCR assay for the detection of measles and rubella virus

Judith M Hübschen  1 , Jacques R Kremer, Sébastien De Landtsheer, Claude P Muller
Affiliations
Comparative Study

A multiplex TaqMan PCR assay for the detection of measles and rubella virus

Judith M Hübschen et al. J Virol Methods. 2008 May.

Abstract

Measles and rubella virus cause fever/rash diseases that are difficult to differentiate clinically. Both viruses can be detected in the same clinical specimens and are propagated on the same cell cultures. A single-tube multiplex TaqMan assay is described for the simultaneous and rapid detection of the full spectrum of known genetic variants. The performance of the assay is similar to a conventional nested PCR and generates cDNA with random primers which can be used directly for virus genotyping.

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