Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2008 Mar 25;105(12):4533-4.
doi: 10.1073/pnas.0801069105. Epub 2008 Mar 19.

Local knowledge helps determine protein structures

Michael R Gryk  1 , Jeffrey C Hoch
Affiliations
Comment

Local knowledge helps determine protein structures

Michael R Gryk et al. Proc Natl Acad Sci U S A. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1.
Fig. 1.
Structural information derived from chemical shifts is more local than information derived from NOEs. (Left) NOEs observed between the same pair of residues, depicted as arrows between the Hα atom of one residue and the Hβ atom (green) and methyl group (yellow) of a “distant” residue, are highly correlated and long-range in their impact: each provides approximately the same structural information, highly constraining the values of all 10 intervening dihedral angles (magenta). (Right) In contrast, chemical shift measurements for the atoms depicted in green provide more local structural constraints for the adjoining dihedral angles. The figure was prepared by using VMD (15).
See this image and copyright information in PMC

Comment on

  • Consistent blind protein structure generation from NMR chemical shift data.
    Shen Y, Lange O, Delaglio F, Rossi P, Aramini JM, Liu G, Eletsky A, Wu Y, Singarapu KK, Lemak A, Ignatchenko A, Arrowsmith CH, Szyperski T, Montelione GT, Baker D, Bax A. Shen Y, et al. Proc Natl Acad Sci U S A. 2008 Mar 25;105(12):4685-90. doi: 10.1073/pnas.0800256105. Epub 2008 Mar 7. Proc Natl Acad Sci U S A. 2008. PMID: 18326625 Free PMC article.

References

    1. Noggle JH, Shirmer RE. The Nuclear Overhauser Effect: Chemical Applications. New York: Academic; 1971.
    1. Connolly PJ, Stern AS, Hoch JC. J Am Chem Soc. 1994;116:2675–2676.
    1. Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, Weissig H, Shindyalov IN, Bourne PE. Nucleic Acids Res. 2000;28:235–242. - PMC - PubMed
    1. Shen Y, Lange O, Delaglio F, Rossi P, Aramini JM, Liu G, Eletsky A, Wu Y, Singarapu KK, Lemak A, et al. Proc Natl Acad Sci USA. 2008;105:4685–4690. - PMC - PubMed
    1. Clore GM, Gronenborn AM. Proc Natl Acad Sci USA. 1998;95:5891–5898. - PMC - PubMed

LinkOut - more resources