Demonstration of 'cardiac-specific' myosin heavy chain in masticatory muscles of human and rabbit

Abstract

Human and rabbit masticatory muscles were analyzed immuno- and enzyme-histochemically using antibodies specific to 'cardiac' alpha, slow and fast myosin heavy chain isoforms. In human masseter, temporalis, and lateral pterygoid muscle 'cardiac' alpha myosin heavy chain is found in fibres that contain either fast, or fast and slow myosin heavy chain. In rabbit masseter, temporalis and digastric muscles, fibres are present that express 'cardiac' alpha myosin heavy chain either exclusively, or concomitantly with slow myosin heavy chain or fast myosin heavy chain. Our results demonstrate a much broader distribution of 'cardiac' alpha myosin heavy chain than hitherto recognized and these might explain in part the specific characteristics of masticatory muscles. The 'cardiac' alpha myosin heavy chain is only found in skeletal muscles originating from the cranial part of the embryo (including the heart muscle), suggesting that its expression might be determined by the developmental history of these muscles.