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. 1976;51(1-2):107-14.
doi: 10.1007/BF01317839.

Purification and characterization of equine infectious anemia virus

H D Matheka, L Coggins, J N Shively, N L Norcross

Purification and characterization of equine infectious anemia virus

H D Matheka et al. Arch Virol. 1976.

Abstract

EIA virus was purified from equine fetal kidney cell cultures by PEG-precipitation, two sucrose-gradient sedimentations (5-30 per cent) and (25 to 60 per cent) centrifugation, using the immunodiffusion test to follow the procedure. Purified EIA virus had a density (20 degrees C) of 1.162 and a sedimentation constant of S20w=656. electron microscopy revealed a particle of about 100 nm in diameter with a very flexible but usually spherical shape. The dense core may be at various locations inside the membrane bound particle.

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References

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