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. 2008 May;46(5):1861-6.
doi: 10.1128/JCM.00109-08. Epub 2008 Mar 26.

PCR method to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella Isolates from the blood of patients with clinical enteric fever

Haim Levy  1 , Souleymane Diallo, Sharon M Tennant, Sofie Livio, Samba O Sow, Milagritos Tapia, Patricia I Fields, Matthew Mikoleit, Boubou Tamboura, Karen L Kotloff, Rosanna Lagos, James P Nataro, James E Galen, Myron M Levine
Affiliations

PCR method to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella Isolates from the blood of patients with clinical enteric fever

Haim Levy et al. J Clin Microbiol. 2008 May.

Abstract

PCR methodology was developed to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B. One multiplex PCR identifies serogroup D, A, and B and Vi-positive strains; another confirms flagellar antigen "d," "a," or "b." Blinded testing of 664 Malian and Chilean Salmonella blood isolates demonstrated 100% sensitivity and specificity.

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Figures

FIG. 1.
FIG. 1.
Validation of the O grouping and H typing multiplex PCRs using 18 reference enteric fever strains. The sample consisted of two Salmonella serovar Typhi, seven Salmonella serovar Paratyphi A, and nine Salmonella serovar Paratyphi B strains. Crude DNA extracts were tested with the O multiplex PCR (A) and H multiplex PCR (B). (C) The interpretation of the PCR products.
FIG. 2.
FIG. 2.
Validation of the dT fermentation PCR using nine Salmonella serovar Paratyphi B reference strains. The PCR control strains were Salmonella serovar Typhi CVD 908-htrA, Salmonella serovar Paratyphi A ATCC 9150, and Salmonella serovar Paratyphi B 431. The Salmonella serovar Paratyphi B reference strains included five dT fermenters and four dT nonfermenters.
FIG. 3.
FIG. 3.
Detection of rare Salmonella serovar Typhi strains that possess fliC-j by the H multiplex PCR. Salmonella serovar Typhi strains 06-0418, 01-0274, and 2433 possess fliC-j and fljBz66. Salmonella serovar Typhi 95-0344 possesses fliC-j only. PCR control strains were Salmonella serovar Typhi CVD 908-htrA, Salmonella serovar Paratyphi A ATCC 9150, and Salmonella serovar Paratyphi B 431.
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