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Review
. 2008:424:125-35.
doi: 10.1007/978-1-60327-064-9_11.

Radiolabeling for two-dimensional gel analysis

Hélian Boucherie  1 , Aurélie Massoni, Christelle Monribot-Espagne
Affiliations
Review

Radiolabeling for two-dimensional gel analysis

Hélian Boucherie et al. Methods Mol Biol. 2008.

Abstract

Radiolabeling is a highly sensitive method for protein detection, which is easily performed by the incorporation of radioactive amino acids into proteins. This makes radiolabeling a method of choice for visualizing proteins separated on two-dimensional (2-D) gels. This chapter presents protocols to determine in vivo labeling conditions and to label proteins for the comparison of protein samples by means of 2-D gel electrophoresis.

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