Frozen protein arrays

Abstract

This chapter describes the rationale behind and means of construction of inexpensive, low to moderate throughput protein arrays. The method of construction is based on injection of analytes into a block of frozen optimum cutting temperature (OCT), the gel media used for frozen sections, and sectioned on a cryostat. The "array section" is applied to a nitrocellulose pad. Once on nitrocellulose, the array can be utilized in any fashion desired. The analytes can be any biologic sample including peptides, proteins, antibodies, cells, nucleic acids, or any other material that can tolerate freezing. This platform provides investigators a flexible inexpensive easy-to-fashion platform to create multiplex assays both in the number of samples analyzed and in the types of assays.

Fig. 1.

Design, construction, and operation of frozen protein array. Used with permission of Proteomics (1).