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. 1976 Jul 1;157(1):217-20.
doi: 10.1042/bj1570217.

Kinetics of reaction of (nitrotyrosyl)cytochrome c with ligands

A José do Nascimento, K Hishida do Nascimento

Kinetics of reaction of (nitrotyrosyl)cytochrome c with ligands

A José do Nascimento et al. Biochem J. .

Abstract

The reaction of [nitrotyrosyl]cytochrome c with ligands was studied by stopped-flow techniques. At pH 7.0 the reaction with imidazole shows two distinct phases, one fast phase being concentration-dependent and a slow phase being concentration-independent. The results are consistent with the existence of two forms of [nitrotyrosyl]cytochrome c in solutions [Schejter et al. (1970) Biochemistry 9, 5118-5122]; form I, the smaller fraction, seems to be responsible for the slow first-order process.

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