RETRACTED: Cardiac overexpression of alcohol dehydrogenase exacerbates chronic ethanol ingestion-induced myocardial dysfunction and hypertrophy: role of insulin signaling and ER stress

Abstract

This article has been retracted: please see Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). At the request of the University of Wyoming, this article has been retracted. The University of Wyoming's institutional investigation of the work authored by Dr. Jun Ren found evidence of data irregularities in Figures 2, 3 and 4 that affect the reported results and conclusions. All authors have been notified of the retraction of this article.

Fig. 1

(A): IPGTT in FVB and ADH transgenic mice consuming ethanol or control diets for 12 weeks; (B). Insulin-stimulated glucose uptake shown as percent increase from baseline. Mean ± SEM, n = 5–6 mice per group, * p < 0.05 vs. FVB, # p < 0.05 vs. FVB+ETOH.

Fig. 2

Myocardial insulin signaling from FVB and ADH mice with control or without ethanol-containing liquid diet intake. (A). Representative gel blots depicting different proteins in the presence or absence of insulin-stimulation (10 nM); (B). Insulin receptor; (C). insulin receptor phosphorylation (Tyr 1146); (D). IRS-1; (E). Tyrosine phosphorylation of IRS-1; and (F). Serine phosphorylation of IRS-1 (Ser307). Mean ± SEM, n = 7 mice per group, * p < 0.05 vs. Non-insulin FVB group, + p < 0.05 vs. insulin-stimulated FVB group, # p < 0.05 vs. insulin-stimulated FVB+ETOH group.

Fig. 3

Cardiomyocyte insulin signaling from FVB and ADH mice with control or without ethanol-containing liquid diet intake. (A). Representative gel blots depicting expression of Akt, p70s6k, GSK-3β, c-Jun and GATA4 (total and phosphorylated); (B). Ak, pAkt and pAkt/Akt ratio; (C). p70s6k, pp70s6k and pp70s6k/p70s6k ratio; (D). GSK-3β, pGSK-3β and pGSK-3β/GSK-3β ratio; (E). c-Jun, pc-Jun and pc-Jun/c-Jun ratio; (F). GATA4, pGATA4 and pGATA4 /GATA4 ratio. Mean ± SEM, n = 5 mice per group, * p < 0.05 vs. corresponding FVB group, # p < 0.05 vs. corresponding FVB+ETOH group.

Fig. 4

Effect of ADH on chronic ethanol (ETOH) administration-induced change in the ER stress markers eIF2α (A), IRE-1α (B), GRP78 (C) and gadd153 (D). Inset: representative gels using specific antibodies. Mean ± SEM, n = 5–6 mice per group, * p < 0.05 vs. FVB, # p < 0.05 vs. FVB+ETOH.