Efficeint transcription of RNA into DNA by avian sarcoma virus polymerase

Abstract

The DNAase digestion end-product of calf thymus DNA contains oligonucleotides that will function as primers for the efficient transcription into DNA of many naturally-occurring RNA's by purified avian sarcoma virus RNA-directed DNA polymerase. The labeled DNA transcripts so obtained are valuable as probes for molecular hybridization studies. Typical applications of the method include the efficient transcription into DNA of 18 and 28 S rRNA as well as the RNA's of avian sarcoma virus, polio virus, influenza virus, satellite tobacco necrosis virus and tobacco mosaic virus. In addition, when these primers are added to avian sarcoma virus particles that have been partially-disrupted with non-ionic detergent there is 6-fold stimulation of the endogenous RNA-directed DNA synthesis.