Membrane lipid acyl group alterations in cells infected with a temperature-conditional mutant of Rous sarcoma virus

Abstract

The increased percentage of oleic acid and decreased percentage of arachidonic acid which occurs in the lipids of chicken embryo fibroblasts transformed by Rous sarcoma virus was shown to be transformation specific rather than a consequence of virus infection. Cells infected with a temperature conditional mutant of Rous sarcoma virus (RSV-T5) had a normal type fatty acid composition when held at the restrictive temperature of 41 degrees C, but had a transformed type fatty acid composition when held at the permissive temperature of 36 degrees C. However, these acyl group changes occurred slowly in the course of transformation, suggesting that they are not a primary event in the genesis of the transformed phenotype.