doi: 10.1099/00221287-137-11-2627.
Identification of a sterol mutant of Neurospora crassa deficient in delta 14,15-reductase activity
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- PMID: 1838392
- DOI: 10.1099/00221287-137-11-2627
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Identification of a sterol mutant of Neurospora crassa deficient in delta 14,15-reductase activity
J Gen Microbiol.
1991 Nov.
Abstract
A mutant (erg-3) of Neurospora crassa resistant to the polyene antibiotic nystatin was compared with its sensitive, wild-type parent to detect differences in sterol composition using gas chromatography-mass spectrometry. The major sterol in wild-type mycelia, comprising 80% of the total, was ergosterol. The major sterols in mutant mycelia, comprising 86% of the total, were delta 8,14-sterols. It is proposed that the nystatin-resistant strain is unable to synthesize ergosterol because it lacks delta 14,15-reductase activity as a result of a mutation in the erg-3 gene.
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