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. 2008 Jun;46(6):2133-6.
doi: 10.1128/JCM.01512-07. Epub 2008 Apr 2.

Identification of thermotolerant campylobacter species by fluorescence in situ hybridization

Sven Poppert  1 , Michaela Haas, Tatjana Yildiz, Thomas Alter, Edda Bartel, Ursula Fricke, Andreas Essig
Affiliations

Identification of thermotolerant campylobacter species by fluorescence in situ hybridization

Sven Poppert et al. J Clin Microbiol. 2008 Jun.

Abstract

Thermotolerant Campylobacter spp. (Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis) are leading causes of food-borne diarrhea in humans. In this study, the usefulness of fluorescence in situ hybridization (FISH) for the identification of Campylobacter isolates was investigated. A hierarchical FISH probe set that included six group-, genus-, and species-specific probes was developed and evaluated with 12 reference strains and 94 clinical isolates of Campylobacter, Arcobacter, and Helicobacter. FISH correctly identified all isolates to the genus level and detected all thermotolerant Campylobacter isolates. The assay showed high degrees of sensitivity for the identification of C. jejuni (90%), C. coli (97%), C. lari (81%), and C. upsaliensis (100%) to the species level.

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Figures

FIG. 1.
FIG. 1.
C. jejuni reference strain stained with FISH probes. The results obtained with C. jejuni ATCC 33560 are shown. Single slides were each stained simultaneously with the fluorescein isothiocyanate-labeled eubacterial probe (EUB) (green; upper row) and the Cy3-labeled Campylobacter-specific probes (red; lower row). Bars, 5 μm.
See this image and copyright information in PMC

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