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. 2006 Jul-Dec;65(3-4):83-6.

The viral bronchiolites diagnosis in children by PCR multiplex

Cristina Tecu  1 , Evelyne Genetay, Viorel Alexandrescu, Astrid Vabret, F Freymuth
Affiliations
  • PMID: 18389721

The viral bronchiolites diagnosis in children by PCR multiplex

Cristina Tecu et al. Roum Arch Microbiol Immunol. 2006 Jul-Dec.

Abstract

The aim of the study was to determine the etiology of the viral bronchiolites in children by using direct immunofluorescence test and 3 RT-PCR Multiplex (S.Bellau-Pujol) The study was performed on 122 nasal inspirations collected from 3 weeks-6 month old children hospitalizated in the pediatrics service of CH Rouen. The results were that the majority (53%) of bronchiolites in children had like etiology RSV and a lot of these infections had double viral etiology (26% RSV+ Rhinovirus; 2,7% RSV+HMPV and 1% RSV+Coronavirus 229E). An important viral factor which gives bronchiolitis in children is HMPV (11%). We also find respiratory infections with triple viral etiology: RSV+Influenza A virus + Rhinovirus.

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