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Comparative Study
. 2008 Apr 1;64(Pt 4):304-6.
doi: 10.1107/S1744309108006775. Epub 2008 Mar 29.

Crystallization and preliminary crystallographic analysis of a plant type III polyketide synthase that produces benzalacetone

Hiroyuki Morita  1 , Michikazu Tanio, Shin Kondo, Ryohei Kato, Kiyofumi Wanibuchi, Hiroshi Noguchi, Shigetoshi Sugio, Ikuro Abe, Toshiyuki Kohno
Affiliations
Comparative Study

Crystallization and preliminary crystallographic analysis of a plant type III polyketide synthase that produces benzalacetone

Hiroyuki Morita et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. .

Abstract

Benzalacetone synthase (BAS) from Rheum palmatum is a plant-specific type III polyketide synthase that catalyzes the one-step decarboxylative condensation of 4-coumaroyl-CoA with malonyl-CoA to produce the diketide 4-(4-hydroxyphenyl)-but-3-en-2-one. Recombinant BAS expressed in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method. The crystals belong to space group P2(1), with unit-cell parameters a = 54.6, b = 89.6, c = 81.1 A, alpha = gamma = 90.0, beta = 100.5 degrees . Diffraction data were collected to 1.8 A resolution using synchrotron radiation at BL24XU of SPring-8.

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Figures

Figure 1
Figure 1
Proposed pathways for the formation of (a) 4-(4-hydroxyphenyl)-but-3-en-2-one by BAS and (b) naringenin chalcone by CHS.
Figure 2
Figure 2
Crystals of BAS grown by the sitting-drop method.
See this image and copyright information in PMC

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