Screening recombinant DNA libraries: a rapid and efficient method for isolating cDNA clones utilizing the PCR
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- PMID: 1839500
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Screening recombinant DNA libraries: a rapid and efficient method for isolating cDNA clones utilizing the PCR
Biotechniques.
1991 Nov.
Abstract
We describe an expeditious method for the isolation of cDNA clones utilizing PCR-based amplification of target sequences from cDNA libraries. This method is rapid, less labor-intensive and inexpensive when compared with screening libraries with radiolabeled probes. This method can be applied to isolate multiple members of a protein family as well as homologous genes in different species by designing appropriate primers to amplify the most conserved regions. Utilizing this method, a novel reticulocyte CD44 transcript was isolated.
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