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. 2008 Jun;46(6):2074-7.
doi: 10.1128/JCM.01858-07. Epub 2008 Apr 9.

Development and evaluation of a novel multiplex PCR technology for molecular differential detection of bacterial respiratory disease pathogens

Robert Benson  1 , Maria L Tondella, Julu Bhatnagar, Maria da Glória S Carvalho, Jacquelyn S Sampson, Deborah F Talkington, Anne M Whitney, Elizabeth Mothershed, Lesley McGee, George Carlone, Vondguraus McClee, Jeannette Guarner, Sherif Zaki, Surang Dejsiri, K Cronin, Jian Han, Barry S Fields
Affiliations

Development and evaluation of a novel multiplex PCR technology for molecular differential detection of bacterial respiratory disease pathogens

Robert Benson et al. J Clin Microbiol. 2008 Jun.

Abstract

The ResPlex I assay (Qiagen) was designed to amplify and detect DNA of six bacterial respiratory pathogens. This assay was compared with real-time PCR assays based upon the same target sequences for the ability detect the target bacteria by use of both stock strains and specimens from respiratory disease patients. The ResPlex I assay is somewhat less sensitive than real-time PCR assays but offers the advantage of multiple assays in a single reaction.

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References

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