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. 2008 Jun;15(6):1028-30.
doi: 10.1128/CVI.00026-08. Epub 2008 Apr 9.

Enhancement of diagnostic efficiency by a gamma interferon release assay for pulmonary tuberculosis

Kelly Aparecida Kanunfre  1 , Olavo Henrique Munhoz Leite, Max Igor Lopes, Marcelo Litvoc, Antonio Walter Ferreira
Affiliations

Enhancement of diagnostic efficiency by a gamma interferon release assay for pulmonary tuberculosis

Kelly Aparecida Kanunfre et al. Clin Vaccine Immunol. 2008 Jun.

Abstract

This study was designed to examine the use of the QuantiFERON-TB Gold assay as an aid in the diagnosis of active pulmonary tuberculosis (TB) in Brazilian patients. Using the receiver operating characteristic curve, the cutoff was adjusted to >or=0.20 IU/ml. The sensitivity increased to 86%, with 100% specificity. All TB patients with negative sputum smear microscopy and negative culture results were positive using this test.

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Figures

FIG. 1.
FIG. 1.
Individual response of IFN-γ released after stimulation with ESAT-6 and CFP-10 antigens. (a) IFN-γ responses in 29 patients with active pulmonary TB. IFN-γ responses in three patients negative by conventional microbiological tests (b) and in 23 healthy subjects (c). The dotted line represents the cutoff of 0.35 IU/ml recommended by the manufacturer; the dashed line represents the cutoff of 0.20 IU/ml defined in our laboratory by ROC curve analysis. *, data for the antigen (ESAT-6 or CFP-10).
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References

    1. Arend, S. M., S. F. Thijsen, E. M. Leyten, J. J. Bouwman, W. P. Franken, B. F. Koster, F. G. Cobelens, A. J. van Houte, and A. W. Bossink. 2007. Comparison of two interferon-gamma assays and tuberculin skin test for tracing tuberculosis contacts. Am. J. Respir. Crit. Care Med. 175:618-627. - PubMed
    1. Centers for Disease Control and Prevention. 2005. Guidelines for using the QuantiFERON-TB Gold test for detecting Mycobacterium tuberculosis infection, United States. MMWR Recomm. Rep. 54:49-55. - PubMed
    1. Chan, E. D., L. Heifets, and M. D. Iseman. 2000. Immunologic diagnosis of tuberculosis: a review. Tuber. Lung Dis. 80:131-140. - PubMed
    1. Dewan, P. K., J. Grinsdale, and L. M. Kawamura. 2007. Low sensitivity of a whole-blood interferon-γ release assay for detection of active tuberculosis. Clin. Infect. Dis. 44:69-73. - PubMed
    1. Ferrara, G., M. Losi, R. D'Amico, P. Roversi, R. Piro, M. Meacci, B. Meccugni, I. M. Dori, A. Andreani, B. M. Bergamini, C. Mussini, F. Rumpianesi, L. M. Fabbri, and L. Richeldi. 2006. Use in routine clinical practice of two commercial blood tests for diagnosis of infection with Mycobacterium tuberculosis: prospective study. Lancet 367:1328-1334. - PubMed

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