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Review
. 2008 Apr 15;105(15):5653-4.
doi: 10.1073/pnas.0802298105. Epub 2008 Apr 9.

Knockout punches with a fistful of zinc fingers

John H Wilson  1
Affiliations
Review

Knockout punches with a fistful of zinc fingers

John H Wilson. Proc Natl Acad Sci U S A. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

The author declares no conflict of interest.

Figures

Fig. 1.
Fig. 1.
ZFN knockouts. In the absence of a template for HR, ZFN-induced DSBs are fixed by NHEJ, which generates imprecise junctions, including both deletions and additions at the joint.
See this image and copyright information in PMC

Comment on

References

    1. Vasquez KM, Marburger K, Intody Z, Wilson JH. Manipulating the mammalian genome by homologous recombination. Proc Natl Acad Sci USA. 2001;98:8403–8410. - PMC - PubMed
    1. Santiago Y, et al. Targeted gene knockout in mammalian cells by using engineered zinc finger nucleases. Proc Natl Acad Sci USA. 2008;105:5809–5814. - PMC - PubMed
    1. Beumer K, Bhattacharyya G, Bibikova M, Trautman JK, Carroll D. Efficient gene targeting in Drosophila with zinc finger nucleases. Genetics. 2006;172:2391–2403. - PMC - PubMed
    1. Bibikova M, Beumer K, Trautman JK, Carroll D. Enhancing gene targeting with designed zinc finger nucleases. Science. 2003;300:764. - PubMed
    1. Morton J, Davis MW, Jorgensen EM, Carroll D. Induction and repair of zinc-finger nuclease-targeted double-strand breaks in Caenorhabditis elegans somatic cells. Proc Natl Acad Sci USA. 2006;103:16370–16375. - PMC - PubMed

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