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. 2008 Jul-Aug;1784(7-8):983-5.
doi: 10.1016/j.bbapap.2008.03.003. Epub 2008 Mar 19.

The proteome of the human neuroblastoma cell line SH-SY5Y: an enlarged proteome

Kambiz Gilany  1 , Roos Van Elzen, Kim Mous, Edmond Coen, Walter Van Dongen, Stefaan Vandamme, Kris Gevaert, Evy Timmerman, Joël Vandekerckhove, Sylvia Dewilde, Xaveer Van Ostade, Luc Moens
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The proteome of the human neuroblastoma cell line SH-SY5Y: an enlarged proteome

Kambiz Gilany et al. Biochim Biophys Acta. 2008 Jul-Aug.

Abstract

The human neuroblastoma cell line SH-SY5Y (ATCC: CRL-2266) is widely used as a neural cellular model system. The hitherto existing proteome data (115 proteins) are here extended. A total of 1103 unique proteins of this cell line were identified using 2D-LC combined with MALDI-TOF/TOF-MS, SDS-PAGE with nano-LC-MS/MS, N-terminal COFRADIC analysis with nano-LC-MS/MS and 2D-PAGE with MALDI-TOF/TOF-MS peptide mass fingerprinting. The obtained proteome profile of this cell line is discussed.

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