doi: 10.1016/0167-4781(91)90201-v.
Nucleotide sequence of trpE, anthranilate synthase I gene, of Bacillus caldotenax
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- PMID: 1840498
- DOI: 10.1016/0167-4781(91)90201-v
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Nucleotide sequence of trpE, anthranilate synthase I gene, of Bacillus caldotenax
Biochim Biophys Acta.
.
Abstract
trpE gene of Bacillus caldotenax was cloned by complementation of an Escherichia coli tryptophan auxotroph. The trpE encoding a 508 amino acid polypeptide is followed by trpD. S1-nuclease mapping of a trpE in vivo transcript suggested that a promoter preceding the trpE was utilized by E. coli. The deduced amino acid sequence of anthranilate synthase I (ASI) was compared with that of five other bacterials ASIs.
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