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Comparative Study
. 1991;36(1):71-4.
doi: 10.1007/BF02935825.

Influence of repeated lyophilization on the survival of Deinococcus proteolyticus, Micrococcus luteus and Escherichia coli

L Rýznar  1 , V Drásil
Affiliations
Comparative Study

Influence of repeated lyophilization on the survival of Deinococcus proteolyticus, Micrococcus luteus and Escherichia coli

L Rýznar et al. Folia Microbiol (Praha). 1991.

Abstract

Repeated lyophilization of Deinococcus proteolyticus, Micrococcus luteus and Escherichia coli cells results in a successive decrease of their survival. The survival curve is exponential with E. coli and M. luteus, and sigmoidal with a broad shoulder with D. proteolyticus both after repeated lyophilization and after UV- or gamma-irradiation. When cells were subjected to gamma-irradiation after a 20-fold freeze-drying, the corresponding survival curve became exponential without the shoulder. Hence we assume that irradiation and repeated lyophilization afflict the same cellular structures and/or functions.

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