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Review
. 2009 Jun;13(6):1043-58.
doi: 10.1111/j.1582-4934.2008.00347.x. Epub 2008 Apr 15.

Mouse models with human immunity and their application in biomedical research

Baojun Zhang  1 , Ziyuan Duan, Yong Zhao
Affiliations
Review

Mouse models with human immunity and their application in biomedical research

Baojun Zhang et al. J Cell Mol Med. 2009 Jun.

Abstract

Biomedical research in human beings is largely restricted to in vitro studies that lack complexity of a living organism. To overcome this limitation, humanized mouse models are developed based on immunodeficient characteristics of severe combined immunodeficiency (SCID) or recombination activating gene (Rag)(null) mice, which can accept xenografts. Peripheral constitution of human immunity in SCID or Rag(null) mice has been achieved by transplantation of mature human immune cells, foetal human thymus, bone marrow, liver tissues, lymph nodes or a combination of these, although efficiency needs to be improved. These mouse models with constituted human immunity (defined as humanized mice in the present text) have been widely used to investigate the basic principles of human immunobiology as well as complex pathomechanisms and potential therapies of human diseases. Here, elements of an ideal humanized mouse model are highlighted including genetic and non-genetic modification of recipient mice, transplantation strategies and proposals to improve engraftments. The applications of the humanized mice to study the development and response of human immune cells, human autoimmune diseases, virus infections, transplantation biology and tumour biology are reviewed as well.

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Figures

Figure 1
Figure 1
The frequencies of cell leakiness in various immunodeficient mouse strains with aging.
Figure 2
Figure 2
The different efficiency of engrafting human HSCs or immune cells in various immunodeficient mouse recipients.
See this image and copyright information in PMC

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