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Comparative Study
. 2008 Apr;57(2):145-8.
doi: 10.1538/expanim.57.145.

Direct comparison between ICSI-mediated DNA transfer and pronuclear DNA microinjection for producing transgenic rats

Masumi Hirabayashi  1 , Megumi Kato, Kazue Amemiya, Shinichi Hochi
Affiliations
Free article
Comparative Study

Direct comparison between ICSI-mediated DNA transfer and pronuclear DNA microinjection for producing transgenic rats

Masumi Hirabayashi et al. Exp Anim. 2008 Apr.
Free article

Abstract

Production efficiency of transgenic rats was compared directly between the routine pronuclear microinjection of exogenous DNA solution (PNMI-Tg method) and the ooplasmic injection of sperm cells exposed to exogenous DNA solution (ICSI-Tg method) using six DNA constructs. The overall production efficiency per treated oocyte in the ICSI-Tg method (mean 1.1%, range 0.2 to 3.1%) was similar to that in the PNMI-Tg method (mean 1.1%, range 0 to 2.4%). An advantage of the ICSI-Tg method in the production of transgenic rats is noted in cases in which a low yield of pronuclear zygotes is an inevitable fate of the rat strain.

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