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. 2008 Jun;74(12):3908-11.
doi: 10.1128/AEM.02928-07. Epub 2008 Apr 18.

Biphenyl dioxygenase from an arctic isolate is not cold adapted

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Biphenyl dioxygenase from an arctic isolate is not cold adapted

Emma R Master et al. Appl Environ Microbiol. 2008 Jun.

Abstract

Biphenyl dioxygenase from the psychrotolerant bacterium Pseudomonas sp. strain Cam-1 (BPDO(Cam-1)) was purified and found to have an apparent k(cat) for biphenyl of 1.1 +/- 0.1 s(-1) (mean +/- standard deviation) at 4 degrees C. In contrast, BPDO(LB400) from the mesophile Burkholderia xenovorans LB400 had no detectable activity at this temperature. At 57 degrees C, the half-life of the BPDO(Cam-1) oxygenase was less than half that of the BPDO(LB400) oxygenase. Nevertheless, BPDO(Cam-1) appears to be a typical Pseudomonas pseudoalcaligenes KF707-type dioxygenase.

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Figures

FIG. 1.
FIG. 1.
Effects of increasing temperature on the biphenyl-degrading activities of ISPCam-1 (♦) and ISPLB400 (□) (n = 3; error bars indicate standard deviations). krel, relative kcat.

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