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. 2008 May;237(5):1477-89.
doi: 10.1002/dvdy.21547.

Spatiotemporal protein distribution of TGF-betas, their receptors, and extracellular matrix molecules during embryonic tendon development

Catherine K Kuo  1 , Bryan C Petersen, Rocky S Tuan
Affiliations

Spatiotemporal protein distribution of TGF-betas, their receptors, and extracellular matrix molecules during embryonic tendon development

Catherine K Kuo et al. Dev Dyn. 2008 May.

Abstract

Tendon is one of the least understood tissues of the musculoskeletal system in terms of development and morphogenesis. Collagen fibrillogenesis has been the most studied aspect of tendon development, focusing largely on the role of matrix molecules such as collagen type III and decorin. While involvement of matrix molecules in collagen fibrillogenesis during chick tendon development is well understood, the role of growth factors has yet to be elucidated. This work examines the expression patterns of transforming growth factor (TGF) -beta1, -beta2, and -beta3, and their receptors with respect to expression patterns of collagen type III, decorin, and fibronectin. We focus on the intermediate stages of tendon development in the chick embryo, a period during which the tendon micro- and macro-architecture are being established. Our findings demonstrate for the first time that TGF-beta1, -beta2, and -beta3 have distinct spatiotemporal developmental protein localization patterns in the developing tendon and strongly suggest that these isoforms have independent roles in tendon development.

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Figures

Figure 1
Figure 1
Schematic of tendon architecture.
Figure 2
Figure 2
Histology of intermediate tendon at developmental days 13,14, 15, and 16. (Top) Hematoxylin-eosin staining; (Bottom) Mallory's trichrome staining. Low magnification, Bar = 200 μm; high magnification, Bar = 50 μm.
Figure 3
Figure 3
Immunohistochemical localization of collagen type III, decorin, and fibronectin in intermediate tendon at developmental days 13 and 14. Low magnification, Bar = 200 μm; high magnification, Bar = 50 μm.
Figure 4
Figure 4
Immunohistochemical localization of collagen type III, decorin, and fibronectin in intermediate tendon at developmental days 15 and 16. Low magnification, Bar = 200 μm; high magnification, Bar = 50 μm.
Figure 5
Figure 5
Immunohistochemical localization of TGF-ß1, -ß2, and -ß3, and TGF-ßRI and RII in intermediate tendon at developmental days 13 and 14. Low magnification, Bar = 200 μm; high magnification, Bar = 50 μm.
Figure 6
Figure 6
Immunohistochemical localization of TGF-ß1, -ß2, and -ß3, and TGF-ßRI and RII in intermediate tendon at developmental days 15 and 16. Low magnification, Bar = 200 μm; high magnification, Bar = 50 μm.
Figure 7
Figure 7
Location of intermediate tendon (red circle) of day 14 chick embryo.
See this image and copyright information in PMC

References

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