Review
doi: 10.1002/0471142727.hga03gs51.
Techniques for mammalian cell tissue culture
Affiliations
- PMID: 18428384
- DOI: 10.1002/0471142727.hga03gs51
Item in Clipboard
Review
Techniques for mammalian cell tissue culture
Curr Protoc Hum Genet.
2006 Nov.
Abstract
This appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
Similar articles
-
Techniques for mammalian cell tissue culture.Curr Protoc Cytom. 2006 May;Appendix 3:Appendix 3B. doi: 10.1002/0471142956.cya03bs36. Curr Protoc Cytom. 2006. PMID: 18770828
-
Techniques for mammalian cell tissue culture.Curr Protoc Mol Biol. 2006 May;Appendix 3:Appendix 3F. doi: 10.1002/0471142727.mba03fs74. Curr Protoc Mol Biol. 2006. PMID: 18265370
-
Techniques for mammalian cell tissue culture.Curr Protoc Neurosci. 2007 Jan;Appendix 3:Appendix 3B. doi: 10.1002/0471142727.nsa03bs38. Curr Protoc Neurosci. 2007. PMID: 18428653
-
Basic Techniques in Mammalian Cell Tissue Culture.Curr Protoc Toxicol. 2016 Nov 1;70:A.3B.1-A.3B.22. doi: 10.1002/cptx.13. Curr Protoc Toxicol. 2016. PMID: 27801934 Review.
-
Avoid Excessive Oxygen Levels in Experiments with Organisms, Tissues and Cells.Adv Microb Physiol. 2015;67:293-314. doi: 10.1016/bs.ampbs.2015.09.001. Epub 2015 Oct 21. Adv Microb Physiol. 2015. PMID: 26616520 Review.
Cited by
-
Understanding cell culture dynamics: a tool for defining protocol parameters for improved processes and efficient manufacturing using human embryonic stem cells.Bioengineered. 2021 Dec;12(1):979-996. doi: 10.1080/21655979.2021.1902696. Bioengineered. 2021. PMID: 33757391 Free PMC article.
-
The importance of cell culture parameter standardization: an assessment of the robustness of the 2102Ep reference cell line.Bioengineered. 2021 Dec;12(1):341-357. doi: 10.1080/21655979.2020.1870074. Bioengineered. 2021. PMID: 33380247 Free PMC article.
-
A genome-wide RNAi screen for polypeptides that alter rpS6 phosphorylation.Methods Mol Biol. 2012;821:187-214. doi: 10.1007/978-1-61779-430-8_11. Methods Mol Biol. 2012. PMID: 22125066 Free PMC article.
-
Fabrication and evaluation of an optimized xenogenic decellularized costal cartilage graft: preclinical studies of a novel biocompatible prosthesis for rhinoplasty.Regen Biomater. 2021 Sep 16;8(6):rbab052. doi: 10.1093/rb/rbab052. eCollection 2021 Oct. Regen Biomater. 2021. PMID: 34584748 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
