Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2006 Nov:Appendix 3:Appendix 3G.
doi: 10.1002/0471142727.hga03gs51.

Techniques for mammalian cell tissue culture

Mary C Phelan  1
Affiliations
Review

Techniques for mammalian cell tissue culture

Mary C Phelan. Curr Protoc Hum Genet. 2006 Nov.

Abstract

This appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.

PubMed Disclaimer

LinkOut - more resources