Review
doi: 10.1002/0471142905.hg0517s49.
Selective isolation of mammalian genes by TAR cloning
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- PMID: 18428393
- DOI: 10.1002/0471142905.hg0517s49
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Review
Selective isolation of mammalian genes by TAR cloning
Curr Protoc Hum Genet.
2006 May.
Abstract
Transformation-associated recombination (TAR) cloning provides a unique tool for selective isolation of desired chromosome segments and full-size genes from complex genomes in the form of a circular yeast artificial chromosome (YAC) up to 250 kb in size. The method has a broad application for structural and functional genomics, long-range haplotyping, mutational analysis of gene families, characterization of chromosomal rearrangements, and evolutionary studies. This unit describes a procedure for gene isolation by TAR as well as a method for conversion of YAC-TAR isolates into a bacterial artificial chromosome (BAC) form.
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