Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2001 May:Chapter 2:Unit 2.7.
doi: 10.1002/0471142700.nc0207s04.

Deoxyribonucleoside phosphoramidites

A Wilk  1 , A Grajkowski, M K Chmielewski, S L Beaucage, L R Phillips
Affiliations

Deoxyribonucleoside phosphoramidites

A Wilk et al. Curr Protoc Nucleic Acid Chem. 2001 May.

Abstract

The detailed preparation of deoxyribonucleoside phosphoramidites bearing a 4-[N-methyl-N-(2,2,2-trifluoroacetyl)amino]butyl group for P(III) protection is presented. The use of this group circumvents nucleobase alkylation during oligonucleotide deprotection. Two syntheses of phosphoramidites starting from either a phosphordichloridite precursor or a bis-(N,N-diisopropylamino)chlorophosphine intermediate are described for the phosphinylation of suitably protected deoxyribonucleosides.

PubMed Disclaimer

MeSH terms

LinkOut - more resources