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. 2001 May:Chapter 6:Unit 6.1.
doi: 10.1002/0471142700.nc0601s00.

Probing RNA structure with chemical reagents and enzymes

W A Ziehler  1 , D R Engelke
Affiliations

Probing RNA structure with chemical reagents and enzymes

W A Ziehler et al. Curr Protoc Nucleic Acid Chem. 2001 May.

Abstract

This unit provides thorough coverage of the most useful chemical and enzyme probes that can be used to examine RNA secondary and tertiary structure. Footprinting methods are presented using dimethyl sulfate, diethyl pyrocarbonate, ethylnitrosourea, kethoxal, CMCT, and nucleases. For chemical probes, both strand scission and primer extension detection protocols are included.

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