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. 2001 May:Appendix 4:Appendix 4B.
doi: 10.1002/0471140864.psa04bs13.

Growth in liquid or solid media

K L Elbing  1 , R Brent
Affiliations

Growth in liquid or solid media

K L Elbing et al. Curr Protoc Protein Sci. 2001 May.

Abstract

This appendix presents basic procedures for growing overnight (and larger) cultures, monitoring growth, and titering and isolating bacterial cultures by serial dilution. In addition, protocols are provided for isolating single colonies by streaking and spreading a plate. Replica plating and strain storage/retrieval is also detailed.

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