doi: 10.1002/0471140864.ps1007s00.
Electroblotting from polyacrylamide gels
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- PMID: 18429098
- DOI: 10.1002/0471140864.ps1007s00
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Electroblotting from polyacrylamide gels
Curr Protoc Protein Sci.
2001 May.
Abstract
This unit contains procedures for electrophoretically transferring proteins onto a variety of membranes including polyvinylidene difluoride (PVDF) and nitrocellulose, and derivatized membranes. The choice of membrane type for electrotransfer is dependent on the ultimate application for the blot membrane. An alternate protocol is provided for electroblotting in semidry systems. This unit also describes procedures for eluting proteins from membranes using detergents or acidic extraction with organic solvents.
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