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. 2001 May:Chapter 10:Unit 10.7.
doi: 10.1002/0471140864.ps1007s00.

Electroblotting from polyacrylamide gels

J A Ursitti  1 , J Mozdzanowski, D W Speicher
Affiliations

Electroblotting from polyacrylamide gels

J A Ursitti et al. Curr Protoc Protein Sci. 2001 May.

Abstract

This unit contains procedures for electrophoretically transferring proteins onto a variety of membranes including polyvinylidene difluoride (PVDF) and nitrocellulose, and derivatized membranes. The choice of membrane type for electrotransfer is dependent on the ultimate application for the blot membrane. An alternate protocol is provided for electroblotting in semidry systems. This unit also describes procedures for eluting proteins from membranes using detergents or acidic extraction with organic solvents.

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