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. 2001 May:Chapter 8:Unit8.4.
doi: 10.1002/0471140864.ps0804s00.

Hydrophobic-interaction chromatography

R M Kennedy  1
Affiliations

Hydrophobic-interaction chromatography

R M Kennedy. Curr Protoc Protein Sci. 2001 May.

Abstract

This unit discusses the important parameters in designing and optimizing a separation by hydrophobic interaction chromatography (HIC), including preparing the sample and choosing a matrix, column, and buffer. Protocols are provided for packing and testing a column; determining binding and elution conditions; eluting the sample; and cleaning, regenerating, and storing HIC columns.

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