Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2001 May:Chapter 15:15.5.1-15.5.24.
doi: 10.1002/0471142735.im1505s20.

Collagen-induced arthritis

Edward F Rosloniec  1 , Michael Cremer  1 , Andrew Kang  1 , Linda K Myers  2
Affiliations

Collagen-induced arthritis

Edward F Rosloniec et al. Curr Protoc Immunol. 2001 May.

Abstract

Collagen-induced arthritis (CIA) is an experimental autoimmune disease that can be elicited in susceptible strains of rodents (rat and mouse) and nonhuman primates by immunization with type II collagen (CII), the major constituent protein of articular cartilage. Because of the important similarities between CIA and rheumatoid arthritis, this experimental model of autoimmune arthritis has been the subject of extensive investigation in several laboratories. Protocols for CIA are described in this unit for both the mouse model and the rat model. In addition, protocols are included for the purification of CII from bovine articular joints and chicken sternums, for the purification of collagen a1(II) chains, and for the purification of fragments of these chains following cyanogen bromide (CNBr) digestion. The preparation of CII is a time-consuming procedure but is usually required because of the scarcity and expense of commercial sources of purified native CII. In addition, support protocols are provided for assessing the severity of inflammation following CIA and for measuring B and T cell responses to CII.

PubMed Disclaimer

References

Literature Cited

    1. Brand, D.D., Myers, L.K., Whittington, K.B., Stuart, J.M., Kang, A.H., and Rosloniec, E.F. 1994. Characterization of the T cell determinants in the induction of autoimmune arthritis by bovine α1(II)-CB11 in H-2q mice. J. Immunol. 152:3088-3097.
    1. Courtenay, J.S., Dallman, M.J., Dayan, A.D., Martin, A., and Mosedale, B. 1980. Immunisation against heterologous type II collagen induces arthritis in mice. Nature 283:666-668.
    1. Cremer, M.A., Griffiths, M.M., Terato, K., and Kang, A.H. 1994a. Type XI and II collagen-induced arthritis in rats: Characterization of inbred rats for arthritis-susceptibility and immune-responsiveness to types XI and II collagen. Autoimmunity 20:153-161.
    1. Cremer, M.A., Ye, X.J., Terato, K., Owens, S.W., Seyer, J.M., and Kang, A.H. 1994b. Type XI collagen-induced arthritis in the Lewis rat: Characterization of cellular and humoral responses to native types XI, V and II collagen and constituent α-chains. J. Immunol. 153:824-832.
    1. Griffiths, M.M. 1988. Immunogenetics of collagen-induced arthritis in rats. Int. Rev. Immunol. 4:1-5.

Substances

LinkOut - more resources