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. 2001 May:Chapter 6:6.23.1-6.23.14.
doi: 10.1002/0471142735.im0623s22.

Measurement of interleukin 16

David M Center  1 , William W Cruikshank  1 , Nereida A Parada  1 , Thomas Ryan  1 , Arthur C Theodore  1 , Gregory Viglianti  2 , Kaiser G Lim  3 , Peter F Weller  3
Affiliations

Measurement of interleukin 16

David M Center et al. Curr Protoc Immunol. 2001 May.

Abstract

Interleukin 16 (IL-16) is a chemoattractant immunomodulatory cytokine that initiates its cellular responses through interaction with membrane-expressed CD4. The protein may be detected by a number of methods; the choice of protocol will depend on the ultimate object of a particular experiment. The first method presented is the use of ELISA to measure IL-16 in cell culture supernatants or biological fluids. For some applications, such as identification of IL-16 in an unknown fluid or medium or direct assessment of its bioactivity, functional assays of IL-16-induced responses may be more appropriate. The chemotactic effects of IL-16 on CD4+ T cells and its specific inhibition may be measured using anti-IL-16 antibodies; the same approach may also be applied to monocytes or eosinophils. Another effect of IL-16 is the induction of CD25, which can be assayed using immunological staining. Finally, cell cycle progression in target cells can be measured by the incorporation of radiolabeled thymidine and confirmed by inhibition with neutralizing antibody.

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References

Literature Cited
    1. Baier, M., Werner, A., Bannert, N., Metzner, K., and Kurth, R. 1995. HIV suppression by interleukin-16. Nature 378:563-564.
    1. Berman, J.S., Beer, D.J., Cruikshank, W.W., and Center, D.M. 1985. Chemoattractant lymphokines specific for the helper/inducer T-lymphocyte subset. Cell. Immunol. 95:105-112.
    1. Center, D.M. and Cruikshank, W. 1982. Modulation of lymphocyte migration by human lymphokines. I. Identification and characterization of chemoattractant activity for lymphocytes from mitogen-stimulated mononuclear cells. J. Immunol. 128:2563-2568.
    1. Cruikshank, W. and Center, D.M. 1982. Modulation of lymphocyte migration by human lymphokines. II. Purification of a lymphotactic factor (LCF). J. Immunol. 128:2569-2571.
    1. Cruikshank, W.W., Berman, J.S., Theodore, A.C., Bernardo, J., and Center, D.M. 1987. Lymphokine activation of T4+ lymphocytes and monocytes. J. Immunol. 138:3817-3825.
Key References
    1. Cruikshank et al., 1991. See above.
    1. Cruikshank et al., 1994. See above.
    1. Cruikshank et al., 1996. See above.
    1. Center, D.M., Kornfeld, H., and Cruikshank, W.W. 1996. Interleukin 16 and its functions as a CD4 ligand. Immunol. Today 17:476-481.

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