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. 2001 May:Chapter 7:7.30.1-7.30.8.
doi: 10.1002/0471142735.im0730s19.

Isolation of human intestinal mucosal mononuclear cells

Claudio Fiocchi  1 , Kenneth R Youngman  2
Affiliations

Isolation of human intestinal mucosal mononuclear cells

Claudio Fiocchi et al. Curr Protoc Immunol. 2001 May.

Abstract

The aim of this procedure is to obtain large numbers of isolated, viable, and functional mononuclear cells that are representative of the lymphoid population present in the mucosa of the human gastrointestinal tract under physiological and pathological conditions. The basic protocol is based on the use of surgically resected small and large bowel specimens, and consists of two basic stages: (1) a combination of chemical, enzymatic, and mechanical treatments to dissociate intestinal tissue and free the mononuclear cells from the surrounding interstitial framework; and (2) separation, isolation, and purification of viable mucosal lamina propria mononuclear cells from other cellular and amorphous components. The proportion of viable cells obtained can be increased by including extra separation steps using nylon wool columns or Percoll gradients, as described.

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References

Literature Cited
    1. Bland, P.W., Richens, E.R., Britton, D.C., and Lloyd, J.V. 1979. Isolation and purification of human large bowel mucosal lymphoid cells: Effect of separation technique on functional characteristics. Gut 20:1037-1046.
    1. Bull, D.M. and Bookman, M.A. 1977. Isolation and functional characterization of human intestinal mucosal lymphoid cells. J. Clin. Invest. 59:966-974.
    1. Clancy, R. 1976. Isolation and kinetic characteristics of mucosal lymphocytes in Crohn's disease. Gastroenterology 70:177-180.
    1. Fiocchi, C., Battisto, J.R., and Farmer, R.G. 1979. Gut mucosal lymphocytes in inflammatory bowel disease. Isolation and preliminary functional characterization. Dig. Dis. Sci 24:705-717.
    1. Goodacre, R., Davidson, R., Singal, D., and Bienenstock, J. 1979. Morphological and functional characteristics of human intestinal lymphoid cells isolated by a mechanical technique. Gastroenterology 76:300-308.
Key References
    1. Bull and Bookman, 1977. See above.
    1. Fiocchi, C. 1985. Lymphoid cells of the gastrointestinal tract. Isolation procedures. Acta. Chirugica Scand. Suppl. 525:11-23.

MeSH terms

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