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Review
. 2004 Sep:Chapter 12:12.4.1-12.4.13.
doi: 10.1002/0471142735.im1204s62.

Culture of HIV in monocytes and macrophages

Affiliations
Review

Culture of HIV in monocytes and macrophages

Suzanne Crowe et al. Curr Protoc Immunol. 2004 Sep.

Abstract

Monocytes and macrophages constitute important cellular targets for macrophage-tropic (M-tropic) strains of HIV-1 and are believed to play a major role in the pathogenesis of AIDS. This updated unit provides procedures for culturing HIV in these target cells under suspension and adherent cell culture conditions. The unit also provides methods for expanding M-tropic primary isolates of HIV and determining the 50% tissue culture infectious dose (TCID50) of HIV stocks.

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References

Literature Cited

References
    1. Alkhatib, G., Combadiere, C., Broder, C., Feng, Y., Kennedy, P., Murphy, P., and Berger, A. 1996. CC CKR5: A RANTES, MIP-α, MIP-β receptor as a fusion cofactor for macrophage-tropic HIV-1. Science 272:1955-1958.
    1. Biggs, B., Hewish, M., Kent, S., Hayes, K., and Crowe, S. 1995. HIV-1 infection of human macrophages impairs phagocytosis and killing of Toxoplasma gondii. J. Immunol. 154:6132-6139.
    1. Chowdhury, M., Munakata, T., Koyanagi, Y., Arai, S., and Yamamoto, N. 1994. Mycoplasma stimulates HIV-1 expression from acutely and dormantly infected promonocyte/monoblastoid cell lines. Arch. Virol. 139:431-438.
    1. Crowe, S. 1995. Role of macrophages in the pathogenesis of human immunodeficiency virus (HIV) infection. Aust. NZ J. Med. 25:777-783.
    1. Crowe, S., Mills, J., and McGrath, M. 1987. Quantitative immunocytofluorographic analysis of CD4 surface antigen expression and HIV infection of human peripheral blood monocyte/macrophages. AIDS Res. Hum. Retroviruses 3:135-145.
Key References
    1. Biggs et al., 1995. See above.
    1. Crowe et al., 1987. See above.

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