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. 2007 Feb:Chapter 7:7.22.1-7.22.4.
doi: 10.1002/0471142735.im0722s76.

Generation of Epstein-Barr Virus (EBV)-immortalized B cell lines

Giovanna Tosato  1 , Jeffrey I Cohen  2
Affiliations

Generation of Epstein-Barr Virus (EBV)-immortalized B cell lines

Giovanna Tosato et al. Curr Protoc Immunol. 2007 Feb.

Abstract

Immortalization of B lymphocytes by EBV is an effective procedure for inducing long-term growth of certain human B lymphocytes. The protocol described in this unit to accomplish this can be divided into three stages: preparation of virus, preparation of target cells to be immortalized, and EBV infection and growth of infected cells.

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References

Literature Cited
    1. Aman, P., Ehlin-Henriksson, B., and Klein, G. 1984. Epstein-Barr virus susceptibility of normal human B lymphocyte populations. J. Exp. Med. 159:208-220.
    1. Chang, I.C., Wu, J.Y., Lu, H.I., Ko, H.W., Kuo, J.L., Wang, C.Y., Shen, P.S., and Hwang, S.M. 2006. High-potentiality preliminary selection criteria and transformation time-dependent factors analysis for establishing Epstein-Barr virus-transformed human lymphoblastoid cell lines. Cell Prolif. 39:457-469.
    1. Elliott, J., Coulter-Mackie, M.B., Jung, J.H., Rodenhiser, D.I., and Singh, S.M. 1991. A method of transforming lymphocytes from very small blood volumes suitable for pediatric samples. Hum. Genet. 86:615-616.
    1. Miller, G. and Lipman, M. 1973. Release of infectious Epstein Barr virus by transformed marmoset leukocytes. Proc. Natl. Acad. Sci. U.S.A. 70:190-194.
    1. Neitzel, H. 1986. A routine method for establishment of permanent growing lymphoblastoid cell lines. Hum. Genet. 73:320-326.
Key Reference
    1. Neitzel, 1986. See above.

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