Methods for the analysis of hard dental tissues exposed to high temperatures

Abstract

The preservation of teeth that have been exposed to a heat source is a key factor in order to keep their value as evidence. This research paper presents some methods under development for the analysis of dental evidence after exposure to high temperatures; it also establishes age related alterations of young and aged teeth which have been exposed to direct heat. A total of thirty permanent healthy teeth (young=15 and old=15) were submitted to direct heat (n=14) and to a controlled raise of temperature (n=16); they were embedded in polymethylmetacrilate. For the macroscopic study, blocks were cut in half, polished and their surfaces were digitalized. Subsequently, blocks were put in slides, worn away and observed through a photomicroscope. The study of the colorimetric pattern was done by Corel Photo Paint 12.0 and Image J. software. All teeth submitted to the gradual raise of temperature presented a degree of structural damage lower than those exposed to direct heat. The internal surface of young teeth exposed to direct heat showed lines of fracture oblique to the long axis of the tooth; in contrast, a reticular pattern was observed on aged teeth. All teeth exposed to direct heat showed some matte-black coloration areas, while those submitted to a gradual increment of temperature showed a greyish colour in the enamel and a chalky-white in the cementum. By using both thermal treatments in young teeth, the amelodentinal junction was lost, thus preserving the integrity of each tissue. In aged teeth, the separation of this junction was produced because of the breakdown of the dentine. Microscopically, in teeth exposed to direct heat, it was neither possible to differentiate the dentin from the cementum, nor to distinguish the incremental lines of the latter; while in those submitted to a controlled increment of temperature, the corresponding morphology in the tissues was observed. A significant statistical difference was obtained for black colour among young and aged teeth exposed to direct heat, as well as among the means of all colours obtained from the teeth exposed to both treatments. The structural changes observed allow locating a tooth in the studied age groups. Our results show the viability of the applied method for the processing of samples, allowing their preservation as evidence during an indefinite time.