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. 2008 Jun;46(6):2088-91.
doi: 10.1128/JCM.00126-08. Epub 2008 Apr 23.

Quality control assessment of human immunodeficiency virus type 2 (HIV-2) viral load quantification assays: results from an international collaboration on HIV-2 infection in 2006

Florence Damond  1 , Antoine Benard, Jean Ruelle, Abraham Alabi, Bernd Kupfer, Perpetua Gomes, Berta Rodes, Jan Albert, Jürg Böni, Jeremy Garson, Bridget Ferns, Sophie Matheron, Geneviève Chene, Françoise Brun-Vezinet; ACHIEV2E Collaboration on HIV-2 Infection Study Group
Collaborators, Affiliations

Quality control assessment of human immunodeficiency virus type 2 (HIV-2) viral load quantification assays: results from an international collaboration on HIV-2 infection in 2006

Florence Damond et al. J Clin Microbiol. 2008 Jun.

Abstract

Human immunodeficiency virus type 2 (HIV-2) RNA quantification assays used in nine laboratories of the ACHI(E)V(2E) (A Collaboration on HIV-2 Infection) study group were evaluated. In a blinded experimental design, laboratories quantified three series of aliquots of an HIV-2 subtype A strain, each at a different theoretical viral load. Quantification varied between laboratories, and international standardization of quantification assays is strongly needed.

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Figures

FIG. 1.
FIG. 1.
Accuracy of quantification assays evaluated by the ACHIEV2E Collaboration in 2006. Quantification results are reported for each participating laboratory. The accuracy interval is represented by the white area for each of the three theoretical viral loads used. Panels: A, theoretical viral load = 3.0 log10 copies/ml; B, theoretical viral load = 2.3 log10 copies/ml; C, theoretical viral load = 1.7 log10 copies/ml.
See this image and copyright information in PMC

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