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. 2008 Aug 15;871(2):271-8.
doi: 10.1016/j.jchromb.2008.04.001. Epub 2008 Apr 8.

Automated SPE-RP-HPLC fractionation of biofluids combined to off-line NMR spectroscopy for biomarker identification in metabonomics

Serge Rezzi  1 , Francia Arce Vera, François-Pierre J Martin, Serena Wang, Dennis Lawler, Sunil Kochhar
Affiliations

Automated SPE-RP-HPLC fractionation of biofluids combined to off-line NMR spectroscopy for biomarker identification in metabonomics

Serge Rezzi et al. J Chromatogr B Analyt Technol Biomed Life Sci. .

Abstract

NMR-based metabonomics is a valuable and straightforward approach to measuring hundreds of metabolites in complex biofluids. However, metabolite identification is sometimes limited by overlapped signals in NMR spectra. We describe a new methodology using an automated hyphenation of solid phase extraction (SPE) with RP-HPLC combined to NMR spectroscopy, which allowed identification of 72 metabolites of various molecular classes in human urine. This methodology was also successfully applied to the fractionation of a cat urine sample to aid identification of aromatic compounds and felinine. The SPE-RP-HPLC method appears to be a reliable tool to support biomarker discovery in metabonomic studies.

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