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Comment
. 2008 May;5(5):383-4.
doi: 10.1038/nmeth0508-383.

BAC to the future: functional genomics in mammals

Assen Roguev, Nevan J Krogan
Comment

BAC to the future: functional genomics in mammals

Assen Roguev et al. Nat Methods. 2008 May.

Abstract

A high-throughput pipeline to engineer bacterial artificial chromosomes (BACs) expressing tagged genes of higher eukaryotes allows large-scale protein localization and interaction studies.

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Figures

Figure 1
Figure 1
A schematic of the BAC TransgeneOmics pipeline. A BAC library is rapidly modified (a modular tag is added in this case). After introduction into cells, protein function can be interrogated in high throughput.
See this image and copyright information in PMC

Comment on

  • BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals.
    Poser I, Sarov M, Hutchins JR, Hériché JK, Toyoda Y, Pozniakovsky A, Weigl D, Nitzsche A, Hegemann B, Bird AW, Pelletier L, Kittler R, Hua S, Naumann R, Augsburg M, Sykora MM, Hofemeister H, Zhang Y, Nasmyth K, White KP, Dietzel S, Mechtler K, Durbin R, Stewart AF, Peters JM, Buchholz F, Hyman AA. Poser I, et al. Nat Methods. 2008 May;5(5):409-15. doi: 10.1038/nmeth.1199. Epub 2008 Apr 6. Nat Methods. 2008. PMID: 18391959 Free PMC article.

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